The present study describes the screening of 189 CAF marker genes by single cell sequencing, and the construction of a six-gene (CAV1, IGFBP3, PLAC9, PLAU, SELM, and SOD3) prognostic signature to evaluate the influence of CAF marker genes on the prognosis of patients with PAAD. The gene discussed is IGFBP3; the disease is pancreatic adenocarcinoma.