While a significant decrease in percentage of GFP+CD19+ CLL-like cells was noted in the BM, with a similar trend in the lymph node (LN) of mice transplanted with CD19-Raptor KO PKCα-KR cells compared to controls, disease eventually accumulated, resulting in similar disease load in the spleen and blood as observed in the controls (Fig. 4E, F and Supplementary Fig. 7). The gene discussed is PRKCA; the disease is B-cell chronic lymphocytic leukemia.