In this study, the cell viability, cytotoxicity, inflammation, and oxidative stress were investigated on lipopolysaccharide (LPS)‐induced AKI model with KDM2B knockdown, while the regulation of nuclear factor κB (NF‐κB) and AP‐1 pathway was also analyzed, which serve to conclude that KDM2B knockdown reduced cytotoxicity, inflammation, and oxidative stress in LPS‐induced AKI via inhibiting NF‐κB and AP‐1 pathways. Here, KDM2B is linked to acute kidney injury.