In addition, to elucidate the spatiotemporal information of immunosuppressive cells in the real immune microenvironment of PDAC, we confirmed that high expression of BHLHE40 in PDAC tissues was associated with a high infiltration of immunosuppressive cells by IHC assay of tumor immunosuppressive cell-specific markers (IL10, TGF-β, CD56, CD163) in combination with BHLHE40 (Fig. 7R). The gene discussed is NCAM1; the disease is neoplasm.