To pursue an exploration of the underlying upstream mechanism of BHLHE40 upregulation in pancreatic cancer, we conducted a prediction analysis of miRNA-BHLHE40 association by coupling the correlation, expression and survival analysis of miRNA and BHLHE40, and ultimately determined that hsa-miR-15a-5p, has-miR-16-5p and has-miR-454-3p in pancreatic cancer are three upstream miRNAs targeting BHLHE40. The gene discussed is BHLHE40; the disease is familial pancreatic carcinoma.