To determine the effect of PLCD3 knockdown on ESCC cell growth, the downregulation of endogenous PLCD3 levels in highly expressing ECA109 cells using RNAoligo-mediated siRNA and verified the ECA109 cell knockdown efficiency by qPCR and WB test results (Fig. 2a,b), The best two siRNA were then selected for a series of functional assays. The gene discussed is PLCD3; the disease is esophageal squamous cell carcinoma.