To investigate quantitatively how the missense mutant p53 proteins affect cellular phenotypes, we performed cell-based functional assays for measuring major cancer hallmarks: 1) cell viability in absence of growth factors; 2) resistance to apoptosis; 3) cell migration; 4) cell invasion; 5) resistance to anoikis; and 6) changes in mammosphere morphology and polarity. Here, TP53 is linked to cancer.