It has been shown that Fn produces various metabolites including formate, succinic acid, 2-hydroxybutyrate, and amino acids such as glutamic acid, aspartic acid, glycine, isoleucine, leucine, phenylalanine, and valine in vitro when contact with colon cancer tissues, or in vivo (11). The gene discussed is FN1; the disease is malignant colon neoplasm.