To see any difference in Fn loads among subgroups of colorectal cancers, we compared the copy number of Fn among colorectal cancers with L/E, MSS, MSI-H, non-MSI-H, MLH1 hypermethylation, non-MLH1 hypermethylation, BRAFV600E, non-BRAFV600E, KRAS mutation, and non-KRAS mutations using the Kruskal–Wallis test (Fig. 1A). This evidence concerns the gene MLH1 and colorectal cancer.