To investigate the possible role of Trx-1 nuclear translocation in IL-6-mediated EMT, invasion and metastasis of CRC, we used lentiviral transduction to establish SW480 and HT-29 cells stably expressing the control vector, WT-Trx-1 (wild-type Trx-1 gene), MT-Trx-1 (KK81-82EE mutant Trx-1,), or shTrx-1 (shRNA targeting Trx-1). Here, IL6 is linked to colorectal carcinoma.