We also investigated the correlation between Trx-1 and pSTAT3 levels in human CRC tissues by qPCR and IHC, which showed Trx-1 mRNA expression to be associated with that of STAT3 and nuclear staining of Trx-1 to have significant positive correlation with nuclear staining of pSTAT3. The gene discussed is TXN; the disease is colorectal carcinoma.