In the co-culture system, including tumor cell (4T1), CD4+ T cells, and Tim-3+ cDC2/Tim-3- cDC2, the cytokines secreted in the supernatant were analyzed; revealing that the levels of IL-6 and IL-10 were substantially higher in the Tim-3+cDC2 co-culture system and indicating an induced increase of Th2 cells. This evidence concerns the gene CD4 and neoplasm.