To further confirm that other immune cells with high or low Tim-3 expression did not influence the cytotoxicity of CD4+ T cells, an in vitro T-cell priming model was employed involving CD4+ T cells that were co-incubated with CD8+ T, BMDM, and NK (with high or low Tim-3 expression) and co-cultured with tumor cells for 24 h. The gene discussed is CD8A; the disease is neoplasm.