CD4 and neoplasm: We observed that CD4+ T cells migrated significantly when Tim-3-cDC2 were co-cultured with tumor cells, whereas Tim-3+cDC2 co-cultured with tumor cells displayed reduced chemotaxis on CD4+ T. Following the addition of αTim-3, CD4+ T cells showed an enhanced migration ability compared to those cultured with Tim-3+cDC2 (Figure 5E).