(29) 8demonstrated, through knockdown and overexpression of SLFN5, as well as luciferase reporter gene assays and metabolomics analysis, that SLFN5 regulates PTEN transcription, the AKT pathway, and proliferation/apoptosis through ZEB1 mediation, and inhibits purine metabolism, leading to cancer cell death. This evidence concerns the gene AKT1 and cancer.