To assess the effects of TPX2 and TTK depletion on the ability of CRC cells to form colonies under conditions that recapitulate the 3D tumor environment, HCT116 and HT-29 were treated with siRNAs targeting TPX2, TTK, or control siRNAs and were subsequently grown in matrigel under 3D culture conditions. Here, TPX2 is linked to colorectal carcinoma.