Consistent with the results of animal model, oxamate enhanced the cytotoxic effect of CAR-T cells in vitro in 51 and 66 A GSCs (Fig. 5F), and a significant reduction on dead tumor cells was shown after oxamate plus CAR-T combination treatment in CD73 overexpressing compared with vector transfecting cells in all detected cells including 51 A, 66 A, SHG141A and SHG142A (Fig. 5F). This evidence concerns the gene NT5E and neoplasm.