Yin et al. elucidated the long-term outcomes of RFA in patients with HCC with a diameter > 3.0 cm and reported that incomplete tumor ablation, recurrent tumors, and pre-ablation AFP levels ≥ 200 ng/mL were independent unfavorable prognostic factors for HCC ranging from 3.0 to 7.0 cm in diameter3. This evidence concerns the gene AFP and neoplasm.