This suggested to us that TgVEGΔROCY1 and TgVEGΔBFD1 parasites were perhaps persisting in the brain at 4 weeks post-infection in a state more reminiscent of tachyzoites than bradyzoites, although it is still possible that they are surrounded by a cyst wall of some kind that just binds the DBA lectin very poorly. Here, RPS19 is linked to infection.