Immunoprecipitation of MITF from an IGR37 human melanoma cell line engineered to express inducible p300 and HA-MITF (Fig. 2c) so as to partially maintain their relative expression levels revealed an increase in acetyl K206 MITF after induction using doxycycline (Fig. 2d), confirming a role for p300 in MITF acetylation. Here, EP300 is linked to melanoma.