NF1 and neoplasm: While most tumors exhibited mutual exclusivity of EGFR and NF1 alterations across all samples, we resolved one patient tumor, P065 (Fig. 4a, orange box) which displayed intratumoral mutual exclusivity across distinct multiregional samples, with an NF1 truncating mutation specifically occurring in two EGFR wild type samples, and conversely, EGFR amplification in the other two NF1 wild type samples.