The mutual exclusivity of EGFR and NF1 alterations was also validated in three independent HGG datasets: 378 samples from TCGA-GBM (95.6%), 292 samples from the GLASS consortium (92.1%)6 and 94 samples from Wang et al. (85.9%)37 (Supplementary Fig. 4). This evidence concerns the gene NF1 and glioblastoma.