Loss of E-cadherin and gain of N-cadherin is a classical hallmark of EMT in CNC and cancer (Araki et al., 2011; Scarpa et al., 2015; Huang et al., 2016; Cousin, 2017), we therefore, tested the expression of E-cadherin and N-cadherin via quantitative capillary Western blot on protein extracts from stage 17 CNC explants. This evidence concerns the gene CDH1 and cancer.