The β diversity analysis of PCoA showed that the intestinal microbiota structure of AOM/DSS mice was significantly isolated from C57, SR‐B1−/+ and AOM/DSS + SR‐B1−/+ mice, while the intestinal microbiota structure of C57, SR‐B1−/+, and AOM/DSS + SR‐B1−/+ mice was similar. This evidence concerns the gene SCARB1 and infectious otitis media.