The current study showed that T1D led to the downregulation of TJ proteins and upregulation of MLCK, ROCK1, and p-MLC, suggesting that disrupted TJ assembly induced by MLCK/ROCK1-p-MLC signaling pathways may contribute to the downregulation of TJ proteins in the colon in diabetes. The gene discussed is MYLK; the disease is type 1 diabetes mellitus.