Figure 6C shows that the DCM signaling pathway was highly enriched, similar to Figure 6B. Also, in Figure 6C, many signaling pathways, such as calcium, oxytocin, estrogen receptor, GPCR-mediated nutrient sensing, and opioid signaling pathways, had significant negative z-scores, indicating high deactivation. Notably, in both the HIV vs. HIV+PSU and WT+PSU vs. HIV+PSU comparisons, the genes Tnnc1, Tnnt2, and Actc1 were found to be majorly involved in the calcium signaling and dilated cardiomyopathy (DCM) signaling pathways. Gene-to-disease associations are provided in Table S3. This evidence concerns the gene ESR1 and dilated cardiomyopathy.