To distinguish the effect PNGase-F-catalyzed deglycosylation had on DC-SIGN-mediated trans-infection, the overall trans-infectivity signals of deglycosylated and control pseudovirus were normalized to their respective direct-infectivity signals to yield the propensity for trans-infection, analogous to what was described in Figure 4B (Figure S7D). This evidence concerns the gene CD209 and infection.