FOXP3 and neoplasm: To assess whether i.t. inoculation of Ad.P60 affected the content of Tregs in spleen and tumor, we used Foxp3-EGFP reporter C57Bl/6J transgenic mice that were engineered to express an IRESEGFP sequence inserted at the 3′ end of the Foxp3 gene (B6.Cg-Foxp3tm2Tch/J) [45,46].