To determine whether PKR depletion affected the replication of HSV-1, HEp-2 cells were transfected with si-PKR#2 for 24 h, infected with HSV-1 (MOI 10), and collected 24 h post-infection to evaluate the viral DNA and viral genes transcriptional levels by real-time PCR (Figure 4). This evidence concerns the gene EIF2AK2 and infection.