Cabral et al. described that cytokines, such as IFN-γ, are produced by 65–75% of infiltrating cells in the chronic phase, which would maintain an inflammatory response in the absence of amastigotes and without blood parasites [40]; this is consistent with our results, where we observed the presence of lymphocyte infiltrate in the apparent absence of parasitemia and parasitism with the conventional methods. This evidence concerns the gene IFNG and parasitic infectious disease.