NR1H4 and metabolic dysfunction-associated steatotic liver disease: To unveil the underlying molecular pathways responsible for the lipid-lowering attributes of AG within the hepatic tissue of HFD-treated NAFLD mice, we investigated the mRNA expression patterns pertaining to a repertoire of pivotal genes associated with fatty acid metabolism, namely ACC1, SCD1, PPAR-γ, CD36, FXR, and SHP.