CASP9 and colorectal cancer: Therefore, we used Compound C to inhibit AMPK, which revealed that HAs induce apoptosis via the AMPK pathway and the apoptosis signaling pathway, which is triggered by Fas/Fas L. Figure 6 indicates that adding AMPK inhibitors increased the expression of p-Akt/Akt and significantly downregulated apoptosis-related proteins such as Fas L, PARP, AIF, and caspase-9, suggesting that HAs induced apoptosis of colorectal cancer LoVo cells through exogenous and intrinsic pathways.