Meanwhile, colony formation ability was reduced by knockdown of BAP31, and this effect was reversed by LiCl in HCT116 and SW480 cells treated with 5-FU, indicating that BAP31 regulates the 5-FU chemosensitivity of CRC cells through β-catenin signaling (Figure 6B). This evidence concerns the gene BCAP31 and colorectal carcinoma.