Mice of C57BL/6 line were fed a regular chow diet (RCD) with or without C5 supplementation and a high-fat diet (HFD) with or without C5 supplementation, whereas LDLR (−/−) and db/db mice (that were used for the modelling of atherosclerosis and obesity) were fed RCD with or without C5 supplementation only (Figure 2). Here, LDLR is linked to obesity due to melanocortin 4 receptor deficiency.