After the esophageal cancer samples were subdivided into subtypes based on their histological features, EAC (n = 50) manifested higher expression levels of GPER1 (p < 0.001), while the expression levels in ESCC (n = 78) were relatively low compared with those in the normal esophageal samples (n = 20) (p < 0.01), as shown in Figure 4b. This evidence concerns the gene GPER1 and esophageal squamous cell carcinoma.