ANXA5 and melanoma: Another study reported that when the human melanoma cell line SK-MEL-28 was treated with piperine at concentrations of 0, 100, 150, and 200 μM for 24 h, and annexin-V/PI staining was performed, the percentage of apoptotic cells increased to 30% in the group treated with 150 μM piperine and 45% in the group treated with 200 μM piperine, demonstrating the apoptosis-inducing effect of piperine [48].