In the mouse intracranial transplantation tumor experiment, we observed that the intracranial tumors in the POLD4 knockdown group were significantly smaller than those in the control group (Figure 13I,J), while the body weight was noticeably higher than that in the control group (Figure 13K), further supporting the impact of POLD4 on glioma cell proliferation in vivo. The gene discussed is POLD4; the disease is central nervous system cancer.