Notably, although the Brassinin-induced apoptosis in the PC-3 cells was found to occur via the inhibition of the PI3K/Akt/mTOR/S6K1 signaling axis [41], there has been no evidence for the role of glycolysis-related proteins and c-Myc/SIRT1/β-catenin signaling in the Brassinin-induced antitumor effect in prostate cancer to date. This evidence concerns the gene AKT1 and Familial prostate cancer.