To further verify whether the improvement of HIIT and MICT on HFD-induced metabolic disorders is related to the cGAS-STING signaling pathway, we examined the expression of proteins related to the cGAS-STING signaling pathway in the skeletal muscle and the serum levels of inflammatory markers IL-6 and IFN-β in the mice of the HFD, MH, and HH groups. This evidence concerns the gene IFNB1 and metabolic disease.