MKI67 and hepatocellular carcinoma: As shown in Figure 6D, IHC analysis showed that the expression of Ki67 in hepatocytes was significantly higher in 16-month-old HBV-tg mice than in age control mice, but was comparable in paired 4-month-old mice and paired 10-month-old mice, indicating that the enhanced cell proliferation had occurred at 16 months, a stage of tumorigenesis, and implying that the HRD1-p27 axis contributes to HCC progression after 10 months.