No relevant changes in global DNA methylation were observed by Masala et al. in the human neuroblastoma SH-SY5Y cell line overexpressing WT or mutant ALS-linked proteins, including TDP-43, as cited above [58]; however, the brains of ALS patients show a different trend; in fact, altered DNA methylation has been recently observed to occur in human post-mortem CNS tissues from ALS patients using immunohistochemistry. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.