MAPT and tauopathy: Interestingly, compared to our IHC data, we observed low signal on western blotting (Supplementary Figure S2), but upon non-denaturing immunoblotting, immunoreactivity was readily observed in brain lysate from our mouse model of tauopathy (Figure 5a), suggesting the conformational epitope was disrupted by conditions required for SDS-PAGE, which has previously been reported with other conformation-specific tau antibodies using heat and chemical denaturants [54,55,56].