With these cut-off values, FAM83H-Nu expression was significantly associated with age (p = 0.042), tumor stage (p = 0.008), lymph node metastasis (p = 0.007), distant metastatic relapse (p < 0.001), histologic grade (p = 0.007), tubule and gland formation (p = 0.029), nuclear pleomorphism (p = 0.002), and the expression of HER2 (p < 0.001), ER (p = 0.009), PR (p = 0.034), FAM83H-Cy (p < 0.001), and PD1 (p < 0.001) (Supplementary Table S1). Here, ESR1 is linked to metastatic malignant neoplasm in the lymph nodes.