DCAF1 and melanoma: Further, the stable depletion of VprBP with shRNA knockdown almost completely eliminated H2AT120p, and these changes could be rescued by the ectopic expression of VprBP wild-type but not VprBP K194R kinase-dead mutant in G361 and MeWo melanoma cells, as assessed by immunostaining and Western blot analyses (Figure 1B,C and Figure S1A,B).