In fact, Crfr1 knockout mice show an anxiolytic phenotype [24] that indicates that CRFR1 mediates an anxiety-like action; however, region-specific knockdown of Crfr1 in globus pallidus externa caused an increase in anxiety-like behavior that highlighted that the effect of CRFR1 activation is brain region-dependent [25]. This evidence concerns the gene CRHR1 and Anxiety.