Our group has demonstrated that the inhibition of AChE increases the cell proliferation rate and the sphere-forming capacity in the HCC cell lines HepG2 and Huh-7 cells; this effect was potentiated by the addition of ACh or an AChE inhibitor; therefore, the enzyme activity, which has been shown to decrease in liver tumors, plays a significant role in the progression of hepatic tumors because it is not enough to deactivate ACh (Figure 9) [92]. This evidence concerns the gene ACHE and hepatocellular carcinoma.