In the current study, we used formalin-fixed, paraffin-embedded (FFPE) lung cancer tissue sections labeled with a specific antibody against PRMT6 to examine the ability of commercially available software algorithms (HALO software version 3.4 including the MiniNet AI and Nuclei Seg plugins for tissue and nuclear segmentation, respectively) to replicate the IRS obtained manually by two pathologists. This evidence concerns the gene PRMT6 and lung cancer.