Double immunofluorescence staining, using an antibody directed against the peroxisomal transporter ABCD3 (PMP70) to mark peroxisomes and BODIPYTM 493/503 to visualise LDs (Figure 4B), revealed a low extent of co-localisation between peroxisomes and LDs, which was not significantly different between control and X-ALD cells (Figure 4C). This evidence concerns the gene ABCD3 and X-linked adrenoleukodystrophy.