For each of the 4 clusters, notable genes associated with glaucoma, neuroinflammation, or reactive astrocytes were: Cluster 1 (Nrf2, Hspb1, S100β), Cluster 2 (Ptgs2, Clcf1), and Cluster 4 (C2, Tlr4, Lcn2, H2-T23, Thbs1, Il6ra) (Table S2). The gene discussed is TLR4; the disease is glaucoma.