The investigation of changes in the level of circulating EVs, positive for PD1, PD-L1 and uPAR as a function of therapy time have been carried out by using liquid biopsy of the same series of MM patients included in the previously described study, in order to explore their role as monitoring factors of anti-PD1 response as well as biomarkers useful for the selection of MM patients to undergo immunotherapy [8]. This evidence concerns the gene PDCD1 and Miyoshi myopathy.