PDCD1 and melanoma: For the first time, we demonstrated that among the circulating EVs positive for PD1, PD-L1 or uPAR released by melanoma cells or immune cells such as T and B cells, monocytes and DCs, only the percentage of those positive for PD1 and released by melanoma cells was reduced in long RES in function of time, while increased in RES when progressed.