To investigate whether these genes with H4K5acK8ac-preferred promoters regulate glioblastoma stem-like properties, i. e., marker gene expression for stem cells and sphere formation efficiency [51, 52], we disrupted ZNF883, RFX4, KLF11, and ZNF835 by siRNA knockdown in 0316-GSC cells. The gene discussed is ZNF835; the disease is glioblastoma.