By conducting CRISPR-Cas9–mediated genome editing of SE regions, we revealed that certain H4K5acK8ac-preferred SEs were responsible for the expression of associated genes (i.e., MYCN and NFIC) in GSCs (Fig. 7A to C) and the maintenance of glioblastoma stem-like properties (Fig. 7F and G). This evidence concerns the gene NFIC and glioblastoma.