CD4 and type 1 diabetes mellitus: After excluding samples that failed quality control (low sequence coverage/quality, low RNA quality, ambiguous sample identity, etc.), sufficient quality RNA for sequencing and analysis was obtained from memory CD4+/CD25+ T cells (henceforth referred to as Tregs) for a total of 84 subjects (49 T1D cases and 35 controls), and from memory CD4+/CD25− T cells for 105 subjects (53 T1D cases and 52 controls).