To examine the effects of CH on mouse dorsal skin, mRNA expression levels of inflammatory factors (TNF-α/IL-1β), hair growth-promoting factors (IGF-1/VEGF), and extracellular matrix (ECM) production-related factors (Elastin/HAS2) were measured using RT-qPCR (Figure 7, Figure 8 and Figure 9). Here, TNF is linked to cyclic hematopoiesis.