To study the impact of rLEL-Ov-TSPs on the migration of host cells, H69 cholangiocytes and M213 CCA cell lines were exposed to rLEL-Ov-TSP-2, rLEL-Ov-TSP-3, and Ov-ES for 24 hours using a Transwell migration assay (Techasen et al. 2014). Here, THBS3 is linked to cholangiocarcinoma.