Next, we used Neat1 knockout (Neat1−/−) mice and their WT littermates to determine their tumour growth of HCC. Primary liver cancer was established by injecting plasmids mixture encoding pT3‐c‐MYC, pX330‐sg‐p53‐cas9 and pT2‐SB13 transposase into the tail vein of experimental and control group mice as described previously.36, 37. The gene discussed is NEAT1; the disease is liver cancer.