To determine the above mechanism, a rescue experiment was designed by using TGF-β1, and activation of the TGF-β signaling pathway significantly promoted the migration and invasion, and expression of E-cadherin were up-regulated and vimentin expression were decreased in osteosarcoma cells and rescued the effects of DIO3OS-knockdown (Fig. 7C–F). This evidence concerns the gene TGFB1 and osteosarcoma.