Using qRT-PCR, the DIO3OS expression of the control cell line (hFOB 1.19) was less than its expression of osteosarcoma cell lines (HOS, MG-63, U2OS, and SaoS-2) in Fig. 6A. Therefore, these outcomes showed that DIO3OS was up-regulated in osteosarcoma, suggesting high expression of DIO3OS might promote osteosarcoma progression. This evidence concerns the gene DIO3OS and osteosarcoma.