Furthermore, after the combined use of DIO3OS silencing and TGF-β signaling pathway activator (TGF-β1), the results revealed that acting the TGF-β signaling pathway restored the affection of DIO3OS silencing on inhibiting the metastasis, migration, and invasion of osteosarcoma in vivo and in vitro. This evidence concerns the gene DIO3OS and osteosarcoma.